TABLE 6.3

Cell retention devices/options and some important parameters for their use

System

Working

volume

range (L)

Max. cell

conc.

(x 1E06

cells/mL)

Max.

perfusion

rate (L/d)

Cell

debris

removal

Residence

time in

equipment

Single use

Additional

equipment

Cells used

Virus

production

demonstrated

Reference

Spin filter

0.5–500

80

0.5–500

No

n.a.

No

Peristaltic

pump

CHO,

Hybridoma,

BHK21

RABV

[ 32, 54]

HFBR

0.004–0.1

72

288

No

Process time Yes

Duet pump,

incubator,

oxygenator

MDCK,

AGE1.CR,

HEK293,

Huh7.5

IAV, MVA,

HIV, HCV

[ 6, 28, 46, 55]

PBBR

0.5–100

150

200

No

Constant

Yes

Peristaltic

pumps,

controller,

incubator

Vero,

HEK293,

MRC-5,

Huh7.5,

MDCK,

CHO

IAV, SARS-

CoV-2, HCV,

VSV, Ad5,

AAV, IPV,

RABV, HAV,

CHIKV

[ 4, 6, 56– 59]

TFF

0.01–2000

200

100

No

10 s

Yes

Levitronix

pump,

peristaltic

pumps

AGE1.CR,

BHK21,

HEK293,

CHO

IAV, YFV, Ad5,

ZIKV

[ 5, 44, 60]

ATF

0.5–5000

130–360

1200

No

1–2 min

Yes

ATF

controller,

peristaltic

pumps

MDCK,

AGE1.CR,

BHK21,

EB66, CHO,

PBG.PK2.1

IAV, MVA,

ZIKV,

YFV, Ad5

[ 5, 36– 38,

44, 45, 61]

150

Bioprocessing of Viral Vaccines